Human Histone H1.2 - HIST1H1C ELISA Kit

Mfr.No.

E5414Hu

Alternative Names

H1.2;H1C;H1F2;H1s-1;HIST1H1C;Histone H1.2;Histone H1c;Histone H1d;Histone H1s-1;

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21365

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.74ng/L

Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Gene ID	3006
UniProt ID	P16403
Antigen	HIST1H1C

Research Area

Others

Protocol

These standard curves of E5414Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.408	2.5	2.459
	2.592
1200ng/L	1.336	1.429	1.387
	1.521
600ng/L	0.824	0.881	0.84
	0.938
300ng/L	0.39	0.449	0.408
	0.509
150ng/L	0.207	0.211	0.169
	0.214
0ng/L	0.053	0.041	0
	0.03

Precision

We measured random samples of E5414Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	710.53	30.6	4.31
Intra-Assay	2	18	1070.7	36.07	3.37
Intra-Assay	3	18	342.23	17.47	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106776	E5414Hu	48T	$320		Add to Cart
BHE12106776	E5414Hu	96T	$458		Add to Cart

Human Histone H1.2 - HIST1H1C ELISA Kit

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