Human Histo-Biood Group Abo System Transferase

Mfr.No.

E4329hu

Description

This sandwich kit is for the accurate quantitative detection of human Histo-biood Group ABO System Transferase (also known as ABO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A transferase;ABO;B transferase;Fucosylglycoprotein 3-alpha-galactosyltransferase;Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase;Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form;Glycoprotein-fucosylgalactoside alpha-galactosyltransferase;Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase;Histo-blood group A transferase;Histo-blood group ABO system transferase;Histo-blood group B transferase;NAGAT

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

40934

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.092ng/ml

Background

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.

Gene ID	28
UniProt ID	P16442
Antigen	ABO

Research Area

Cardiovascular

Protocol

These standard curves of E4329hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.313	2.235	2.189
	2.156
16ng/ml	1.691	1.595	1.549
	1.499
8ng/ml	1.117	1.063	1.018
	1.009
4ng/ml	0.671	0.715	0.67
	0.76
2ng/ml	0.455	0.428	0.383
	0.401
0ng/ml	0.056	0.045	0
	0.035

Precision

We measured random samples of E4329hu within the same batch/lot to ensure the consistency of the kits' performances.

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105698	E4329hu	48T	$320		Add to Cart
BHE12105698	E4329hu	96T	$458		Add to Cart

Human Histo-Biood Group Abo System Transferase - ABO ELISA Kit

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