Human Histatin-3 - HTN3 ELISA Kit

Mfr.No.

E5407Hu

Alternative Names

Basic histidine-rich protein;His3 1/11;His3 1/12;His3 1/13;His3 1/24;His3 1/25;His3 12/24;His3 12/25;His3 12/32;His3 13/24;His3 13/25;His3 14/24;His3 14/25;His3 15/24;His3 15/25;His3 20/30;His3 20/31;His3 20/32;His3 20/43;His3 20/44;His3 24/30;His3 24/31;His3 24/32;His3 25/30;His3 25/32;His3 26/30;His3 26/31;His3 26/32;His3 28/32;His3 29/32;His3 31/43;His3 31/44;His3 31/51;His3 32/44;His3 32-43;His3 33/43;His3 33/44;His3 34/43;His3 45/51;His3 47/51;His3 34/44;His3 48/51;His3 5/11;His3 5/12;His3 5/13;PB;HTN3;Hst;Histidine-rich protein 3;Histatin-9;Histatin-8;Histatin-7;Histatin-6;Histatin-5;Histatin-4;Histatin-3 7/13;Histatin-3 7/12;Histatin-3 7/11;Histatin-3 6/13;Histatin-3 6/11;Histatin-3 5/13;Histatin-3 5/12;Histatin-3 5/11;Histatin-3 29/32;Histatin-3 28/32;Histatin-3 26/32;Histatin-3 15/25;Histatin-3 15/24;Histatin-3 14/25;Histatin-3 14/24;Histatin-3 13/25;Histatin-3 13/24;Histatin-3 12/32;Histatin-3 12/25;Histatin-3 12/24;Histatin-3 1/25;Histatin-3 1/24;Histatin-3 1/13;Histatin-3 1/12;Histatin-3 1/11;Hist

Reactivity

Human

Detection Range

0.05-20ug/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

6149

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.029ug/mL

Background

Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. His3-(20-43)-peptide (histatin-5) is especially effective against C.albicans and C.neoformans, and inhibits Lys-gingipain and Arg-gingipain (rgpB) from P.gingivalis. In addition, His3-(20-43)-peptide is a potent inhibitor of metalloproteinases MMP2 and MMP9.

Gene ID	3347
UniProt ID	P15516
Antigen	HTN3

Research Area

Others

Protocol

These standard curves of E5407Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ug/mL	2.055	2.178	2.124
	2.301
6ug/mL	1.375	1.373	1.318
	1.37
3ug/mL	0.893	0.831	0.776
	0.768
1.5ug/mL	0.538	0.528	0.473
	0.517
0.75ug/mL	0.224	0.251	0.197
	0.278
0ug/mL	0.064	0.054	0
	0.045

Precision

We measured random samples of E5407Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.1	0.06	5.4
Intra-Assay	2	18	3.6	0.21	5.7
Intra-Assay	3	18	6.3	0.26	4.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106769	E5407Hu	48T	$320		Add to Cart
BHE12106769	E5407Hu	96T	$458		Add to Cart

Human Histatin-3 - HTN3 ELISA Kit

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