Human HGFA ELISA Kit PicoKine®

Mfr.No.

EK1592

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.031	0.107	0.174	0.323	0.584	1.099	1.729	2.371

Description

Human HGFA ELISA Kit PicoKine® (96 Tests). Quantitate Human HGFAC in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatocyte growth factor activator;HGF activator;HGFA;3.4.21.-;Hepatocyte growth factor activator short chain;Hepatocyte growth factor activator long chain;HGFAC;

Molecular Weight

70682 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q36-S655

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HGFA

Subcellular Localization

Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	62	304	830	60	296	836
Standard deviation	3.1	20.06	52.29	3.9	22.79	59.35
CV (%)	5%	6.6%	7.7%%	6.5%	7.7%	7.1%

Protein Function

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

Background

Hepatocyte growth factor activator, also known as HGFA, is a protein that in humans is encoded by the HGFAC gene. This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	62	62	64	63	62	0.82	1.3%
Sample 2	304	296	265	269	283	16.8	5.9%
Sample 3	830	851	870	915	866	31.37	3.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3083
Gene Name	HGFAC
UniProt ID	Q04756
Protein Name	Hepatocyte growth factor activator
Tissue Specificity	Liver.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001275	EK1592	96 wells/kit	$499		Add to Cart

Human HGFA ELISA Kit PicoKine®

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