Human Herpud 1 ELISA Kit

Mfr.No.

E5436Hu

Alternative Names

HERP;HERPUD1;Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein;Methyl methanesulfonate;Mif1;MMF-inducible fragment protein 1;SUP

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

43720

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.16ng/L

Background

Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:16289116, PubMed:28827405). Could enhance presenilin-mediated amyloid-beta protein 40 generation. Binds to ubiquilins and this interaction is required for efficient degradation of CD3D via the ERAD pathway (PubMed:18307982).

Gene ID	9709
UniProt ID	Q15011
Antigen	HERPUD1

Research Area

Others

Protocol

These standard curves of E5436Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.147	2.242	2.218
	2.337
600ng/L	1.218	1.292	1.268
	1.365
300ng/L	0.756	0.679	0.656
	0.602
150ng/L	0.356	0.395	0.372
	0.435
75ng/L	0.199	0.176	0.152
	0.152
0ng/L	0.023	0.024	0
	0.024

Precision

We measured random samples of E5436Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	390.16	19.42	4.98
Intra-Assay	2	18	629.14	20.94	3.33
Intra-Assay	3	18	218.07	12.52	5.74

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106798	E5436Hu	48T	$320		Add to Cart
BHE12106798	E5436Hu	96T	$458		Add to Cart

Human Herpud 1 ELISA Kit

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