Human Hepatocyte Nuclear Factor 1-alpha

Mfr.No.

E6690Hu

Alternative Names

Hepatocyte nuclear factor 1-alpha;HNF1;HNF1A;HNF-1A;HNF1alpha;HNF-1-alpha;HNF4A;IDDM20;LFB1;Liver-specific transcription factor LF-B1;MODY3;TCF1;TCF-1;Transcription factor 1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

67356

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.13ng/L

Background

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:12453420, PubMed:10966642). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Gene ID	6927
UniProt ID	P20823
Antigen	HNF1A

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E6690Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.18	2.202	2.161
	2.225
800ng/L	1.352	1.413	1.372
	1.474
400ng/L	0.72	0.696	0.654
	0.672
200ng/L	0.421	0.382	0.34
	0.342
100ng/L	0.211	0.192	0.15
	0.173
0ng/L	0.038	0.042	0
	0.046

Precision

We measured random samples of E6690Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	412.43	20.43	4.95
Intra-Assay	2	18	881.95	30.36	3.44
Intra-Assay	3	18	370.26	20.94	5.66

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115301	E6690Hu	48T	$320		Add to Cart
BHE12115301	E6690Hu	96T	$458		Add to Cart

Human Hepatocyte Nuclear Factor 1-alpha - HNF1A ELISA Kit

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