Human hepatitis D virus(HDV) antibody(IgG) ELISA Kit
BHE10502693
This Pig GH1 ELISA Kit was designed for the quantitative measurement of Pig GH1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-100 ng/mL and the sensitivity is 2.5 ng/mL.
The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
96
90-102
EDTA plasma (n=4)
98
92-103
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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Sample
Serum(n=4)
1:1
Average %
92
Range %
87-96
1:2
Average %
95
Range %
89-100
1:4
Average %
94
Range %
88-99
1:8
Average %
95
Range %
90-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
0
1.905
1.834
1.870
2.5
1.298
1.282
1.290
7.5
1.024
1.004
1.014
23
0.739
0.722
0.731
50
0.567
0.531
0.549
100
0.342
0.338
0.340
Species
Homo sapiens
Sample Type
Serum, Plasma
Limit Of Detection
450 nm
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.