Human Hemopexin ELISA Kit PicoKine®

Mfr.No.

EK1466

Sensitivity

<50 pg/ml

Detection Range

3.12 ng/ml - 200 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.023	0.042	0.086	0.127	0.256	0.506	0.892	1.820

Description

Human Hemopexin ELISA Kit PicoKine® (96 Tests). Quantitate Human HPX in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hemopexin;Beta-1B-glycoprotein;HPX;

Molecular Weight

51676 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T24-H462

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural human Hemopexin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	7144	26562	114865	7626	24479	107968
Standard deviation	535.8	1089.04	5628.38	571.95	1345.5%4	7125.88
CV (%)	7.5%	4.1%	4.9%	7.5%	5.5%	6.6%

Protein Function

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin retus to the circulation.

Background

Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the tuover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for intealisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	7144	6667	7002	7690	7125	368.94	5.1%
Sample 2	26562	26917	29717	26482	27419	1336.52	4.8%
Sample 3	114865	125689	107714	123178	117861	7097.28	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3263
Gene Name	HPX
UniProt ID	P02790
Protein Name	Hemopexin
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA
Research Area	Cardiovascular,Cofactors, Vitamins/Minerals,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Metabolism,Pathways and Processes,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001081	EK1466	96 wells/kit	$499		Add to Cart

Human Hemopexin ELISA Kit PicoKine®

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