Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (96 Tests). Quantitate Human HJV in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Alternative Names
Hemojuvelin;Hemochromatosis type 2 protein;RGM domain family member C;HFE2;HJV, RGMC;
Molecular Weight
45080 MW
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q36-D400
Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .
Background
Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. In humans, the hemojuvelin protein is encoded by the HFE2 gene. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
Reproducibility
Lots
Lot 1 (pg/ml)
Lot 2 (pg/ml)
Lot 3 (pg/ml)
Lot 4 (pg/ml)
Mean (pg/ml)
Standard Deviation
CV (%)
Sample 1
285
292
319
286
295
13.82
4.6%
Sample 2
1139
1247
1212
1220
1204
39.97
3.3%
Sample 3
5792
6174
5259
6098
5830
359.73
6.1%
*number of samples for each test n=16.
Notes
Material Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer . Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Gene ID
148738
Gene Name
HJV
UniProt ID
Q6ZVN8
Protein Name
Hemojuvelin
Tissue Specificity
Adult and fetal liver, heart, and skeletal muscle. .
Applications
ELISA
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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