Human Hemoglobin Subunit Beta

Mfr.No.

E1121Hu

Sensitivity

0.43ug/ml

Detection Range

1-400ug/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Hemoglobin Subunit Beta (also known as HBB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Beta-globin;HBB;Hemoglobin beta chain;Hemoglobin subunit beta;LVV-hemorphin-7;Spinorphin

Molecular Weight

15998

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1121Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	144.5	4.03	2.79
Intra-Assay	2	18	66.93	3.46	5.17
Intra-Assay	3	18	34.61	2.56	7.4

Background

Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. (Spinorphin): functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.

Protocol

These standard curves of E1121Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240mg/ml	2.123	2.209	2.174
	2.294
120mg/ml	1.332	1.407	1.372
	1.483
60mg/ml	0.74	0.82	0.785
	0.901
30mg/ml	0.451	0.469	0.434
	0.487
15mg/ml	0.242	0.239	0.204
	0.236
0mg/ml	0.036	0.035	0
	0.034

Gene ID	3043
UniProt ID	P68871

Research Area	Others

Storage	2-8°C

Reference	Clusterin serum level: how does it affect psoriatic patients? Journal: J Biol Regul Homeost Agents. 2017 Jul-Sep;31(3):785-789

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102769	E1121Hu	48T	$320		Add to Cart
BHE12102769	E1121Hu	96T	$458		Add to Cart

Human Hemoglobin Subunit Beta - HBB ELISA Kit

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