Human Heat Shock Protein Beta 3

Mfr.No.

E5378Hu

Sensitivity

0.15ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DHMN2C;Heat shock 17 kDa protein;Heat shock protein beta-3;HMN2C;HSP 17;HSPB3;HSPL27;Protein 3

Molecular Weight

16966

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5378Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.14	1	5.53
Intra-Assay	2	18	32.47	0.93	2.86
Intra-Assay	3	18	11.05	0.77	6.96

Background

Heat shock protein beta-3 (HspB3) also known as heat shock 27kDa protein 3 is a protein that in humans is encoded by the HSPB3 gene. This gene encodes a muscle-specific small heat shock protein.

Protocol

These standard curves of E5378Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.172	2.196	2.141
	2.219
24ng/mL	1.247	1.257	1.203
	1.267
12ng/mL	0.678	0.741	0.687
	0.804
6ng/mL	0.462	0.449	0.394
	0.436
3ng/mL	0.324	0.314	0.26
	0.304
0ng/mL	0.059	0.054	0
	0.05

Gene ID	8988
UniProt ID	Q12988

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106741	E5378Hu	48T	$320		Add to Cart
BHE12106741	E5378Hu	96T	$458		Add to Cart

Human Heat Shock Protein Beta 3 - HSPB3 ELISA Kit

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