Human Heat Shock 70 kDa Protein 12B

Mfr.No.

E5373Hu

Sensitivity

0.047ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C20orf60;Heat shock 70 kDa protein 12B;HSPA12B

Molecular Weight

75688

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5373Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.24	0.43	3.86
Intra-Assay	2	18	3.2	0.18	5.73
Intra-Assay	3	18	8.46	0.6	7.04

Background

Heat shock protein family A (Hsp70) member 12B is a protein that in humans is encoded by the HSPA12B gene. The protein encoded by this gene contains an atypical heat shock protein 70 (Hsp70) ATPase domain and is therefore a distant member of the mammalian Hsp70 family. This gene may be involved in susceptibility to atherosclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

Protocol

These standard curves of E5373Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.156	2.245	2.182
	2.334
12ng/ml	1.54	1.442	1.379
	1.344
6ng/ml	0.867	0.87	0.807
	0.873
3ng/ml	0.461	0.447	0.384
	0.434
1.5ng/ml	0.301	0.284	0.221
	0.267
0ng/ml	0.071	0.063	0
	0.055

Gene ID	116835
UniProt ID	Q96MM6

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106736	E5373Hu	48T	$320		Add to Cart
BHE12106736	E5373Hu	96T	$458		Add to Cart

Human Heat Shock 70 kDa Protein 12B - HSPA12B ELISA Kit

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