Matrices listed below were spiked with certain level of HCV IgG and the recovery rates were calculated by comparing the measured value to the expected amount of HCV IgG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
94
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
88-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCV IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.