Human HCG-IgM (Human chorionic gonadotropin-Immunoglobulin M) ELISA Kit

Mfr.No.

EH4768

Sensitivity

Qualitative

Detection Range

Qualitative

Reactivity

Human

Assay Duration

3 hours

Assay Type

Indirect

Description

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HCG-IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HCG-IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alternative Names

HCG-IgM ELISA Kit, Human chorionic gonadotropin-Immunoglobulin M ELISA Kit

Specificity

Specifically binds with HCG-IgM , no obvious cross reaction with other analogues.

Sample Type

Tissue lysate, Cell lysates, Cell culture supernatants, Plasma, Other biological fluids

Assay Procedure Summary

Step 1:
Wash the plate twice before adding the standard and sample.

Step 2:
Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.

Washing:
Wash the plate three times and immerse for 1min each time.

Step 3:
Add 100ul HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times and immerse for 1min each time.

Step 4:
Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 5:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

Specifically binds with HCG-IgM , no obvious cross reaction with other analogues.

Usage Notes

Step 1:
Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.

Washing:
Wash the plate twice without immersion.

Step 2:
Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times without immersion.

Step 3:
Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 4:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Antigen	HCG-IgM

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10810011	EH4768	96T	$455		Add to Cart

Human HCG-IgM (Human chorionic gonadotropin-Immunoglobulin M) ELISA Kit

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