Matrices listed below were spiked with certain level of HbsAg and the recovery rates were calculated by comparing the measured value to the expected amount of HbsAg in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
96
EDTA plasma(n=5)
91-104
98
heparin plasma(n=5)
87-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HbsAg and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HbsAg ELISA Kit allows for the in vitro quantitative determination of HbsAg concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HbsAg(hepatitis B virus Surface Antigen) ELISA Kit