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Fine Test
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Fine Test
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| Mfr.No. | EH4002 | ||||||
| Sensitivity | Qualitative | ||||||
| Detection Range | Qualitative | ||||||
| Reactivity | Human | ||||||
| Assay Duration | 3 hours | ||||||
| Assay Type | Qualitative | ||||||
| Description | This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HbsAg bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HbsAg in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. | ||||||
| Alternative Names | HBsAg ELISA Kit, Hepatitis B virus Surface Antigen ELISA Kit | ||||||
| Specificity | Specifically binds with HbsAg , no obvious cross reaction with other analogues. | ||||||
| Sample Type | Tissue lysate, Cell lysates, Cell culture supernatants, Plasma, Other biological fluids | ||||||
| Components | Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution | ||||||
| Stability |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
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| Notes | For Research Use Only | ||||||
| Usage Notes |
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