Human HBEGF ELISA Kit PicoKine®

Mfr.No.

EK0770

Sensitivity

<10 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.029	0.063	0.099	0.181	0.323	0.643	1.307	2.025

Description

Human HBEGF ELISA Kit PicoKine® (96 Tests). Quantitate Human HBEGF in cell culture supeatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Proheparin-binding EGF-like growth factor;Heparin-binding EGF-like growth factor;HB-EGF;HBEGF;Diphtheria toxin receptor;DT-R;HBEGF;DTR, DTS, HEGFL;

Molecular Weight

23067 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D63-L148

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HBEGF

Subcellular Localization

Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	16	77	282	15	70	280
Standard deviation	1	3.77	11.56	1.05	4.2	11.76
CV (%)	6%	4.9%	4.1%	7%	6%	4.2%

Protein Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Background

HBEGF (Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	16	14	15	15	15	0.7	4.6%
Sample 2	77	73	73	83	76	4.09	5.3%
Sample 3	282	268	286	279	278	6.68	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1839
Gene Name	HBEGF
UniProt ID	Q99075
Protein Name	Proheparin-binding EGF-like growth factor
Sequence	Contains 1 EGF-like domain.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000614	EK0770	96 wells/kit	$499		Add to Cart

Human HBEGF ELISA Kit PicoKine®

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