Matrices listed below were spiked with certain level of HbA1c and the recovery rates were calculated by comparing the measured value to the expected amount of HbA1c in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
89-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HbA1c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.