Human Hairy Enhancer of Split Related with Yrpw Motif-like Protein

Mfr.No.

E5371Hu

Alternative Names

bHLHb33;Class B basic helix-loop-helix protein 33;Hairy/enhancer-of-split related with YRPW motif-like protein;Hairy-related transcription factor 3;HESR3;HEY3;HEYL;hHeyL;hHRT3;HRT3;HRT-3

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

35087

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.93ng/L

Background

Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Gene ID	26508
UniProt ID	Q9NQ87
Antigen	HEYL

Research Area

Others

Protocol

These standard curves of E5371Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.26	2.212	2.16
	2.165
800ng/L	1.224	1.293	1.241
	1.362
400ng/L	0.786	0.732	0.68
	0.678
200ng/L	0.517	0.469	0.417
	0.421
100ng/L	0.197	0.218	0.166
	0.238
0ng/L	0.051	0.052	0
	0.053

Precision

We measured random samples of E5371Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	542.93	30.42	5.6
Intra-Assay	2	18	845.6	30.98	3.66
Intra-Assay	3	18	337.02	21.34	6.33

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106734	E5371Hu	48T	$320		Add to Cart
BHE12106734	E5371Hu	96T	$458		Add to Cart

Human Hairy Enhancer of Split Related with Yrpw Motif-like Protein - HEYL ELISA Kit

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