Human HAI-2/SPINT2 ELISA Kit PicoKine®

Mfr.No.

EK0773

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.040	0.081	0.118	0.209	0.440	0.872	1.400	2.259

Description

Human HAI-2/SPINT2 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPINT2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kunitz-type protease inhibitor 2;Hepatocyte growth factor activator inhibitor type 2;HAI-2;Placental bikunin;SPINT2;HAI2, KOP;

Molecular Weight

28228 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-K197

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HAI-2

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supernatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	54	205	935	56	220	990
Standard deviation	2.91	11.07	61.71	3.3	12.32	65.6
CV (%)	5.4%	5.4%	6.6%	5.9%	5.6%	6.7%

Protein Function

Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.

Background

Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	54	50	48	49	50	2.27	4.5%
Sample 2	205	214	221	199	209	8.4	4%
Sample 3	935	949	806	919	902	56.57	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10653
Antigen	HAI-2/SPINT2
Gene Name	SPINT2
UniProt ID	O43291
Protein Name	Kunitz-type protease inhibitor 2
Tissue Specificity	Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.

Applications	ELISA
Research Area	Cell Biology,Developmental Biology,Neurogenesis,Neurology Process,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Reproduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001118	EK0773	96 wells/kit	$499		Add to Cart

Human HAI-2/SPINT2 ELISA Kit PicoKine®

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