Human HAI-2/SPINT2 ELISA Kit PicoKine®

Mfr.No.

EK0773

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.040	0.081	0.118	0.209	0.440	0.872	1.400	2.259

Description

Human HAI-2/SPINT2 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPINT2 in cell culture supeatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kunitz-type protease inhibitor 2;Hepatocyte growth factor activator inhibitor type 2;HAI-2;Placental bikunin;SPINT2;HAI2, KOP;

Molecular Weight

28228 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-K197

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HAI-2

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	54	205	935	56	220	990
Standard deviation	2.91	11.07	61.71	3.3	12.32	65.6
CV (%)	5.4%	5.4%	6.6%	5.9%	5.6%	6.7%

Protein Function

Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.

Background

Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	54	50	48	49	50	2.27	4.5%
Sample 2	205	214	221	199	209	8.4	4%
Sample 3	935	949	806	919	902	56.57	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10653
Gene Name	SPINT2
UniProt ID	O43291
Protein Name	Kunitz-type protease inhibitor 2
Tissue Specificity	Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.

Applications	ELISA
Research Area	Cell Biology,Developmental Biology,Neurogenesis,Neurology Process,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Reproduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001118	EK0773	96 wells/kit	$499		Add to Cart

Human HAI-2/SPINT2 ELISA Kit PicoKine®

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