Matrices listed below were spiked with certain level of HAAO and the recovery rates were calculated by comparing the measured value to the expected amount of HAAO in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
95
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
89-99
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HAAO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HAAO ELISA Kit allows for the in vitro quantitative determination of HAAO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HAAO(3-hydroxyanthranilate 3,4-dioxygenase) ELISA Kit