Human Growth Differentiation Factor 9

Mfr.No.

E3059Hu

Sensitivity

5.39ng/L

Detection Range

10-4000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Growth Differentiation Factor 9 (also known as GDF9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GDF 9;GDF9;GDF-9;Growth/differentiation factor 9

Molecular Weight

51444

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3059Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	183	9.09	5
Intra-Assay	2	18	902.1	45.8	5.1
Intra-Assay	3	18	498.8	29.25	5.9

Background

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.

Protocol

These standard curves of E3059Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.238	2.177	2.144
	2.115
1200ng/L	1.934	1.886	1.854
	1.839
600ng/L	1.135	1.115	1.083
	1.096
300ng/L	0.748	0.674	0.641
	0.599
150ng/L	0.381	0.393	0.36
	0.405
0ng/L	0.035	0.033	0
	0.031

Gene ID	2661
UniProt ID	O60383

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104593	E3059Hu	48T	$320		Add to Cart
BHE12104593	E3059Hu	96T	$458		Add to Cart

Human Growth Differentiation Factor 9 - GDF9 ELISA Kit

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