Human Growth Differentiation Factor 15

Mfr.No.

E0037Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Growth Differentiation Factor 15 (also known as GDF15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GDF 15;GDF15;GDF-15;Growth/differentiation factor 15;Macrophage inhibitory cytokine 1;MIC-1;NAG-1;NRG-1;NSAID-activated gene 1 protein;NSAID-regulated gene 1 protein;Placental bone morphogenetic protein;Placental TGF-beta;Prostate differentiation factor;

Reactivity

Human

Detection Range

10-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34140

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.57ng/L

Background

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed: 28953886, PubMed: 28846097, PubMed: 28846098, PubMed: 28846099, PubMed: 23468844, PubMed: 29046435). Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed: 28953886, PubMed: 28846097, PubMed: 28846098, PubMed: 28846099). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed: 28953886). On hepatocytes, inhibits growth hormone signaling (By similarity).

Gene ID	9518
UniProt ID	Q99988
Antigen	GDF15

Research Area

Signal Transduction

Protocol

These standard curves of E0037Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.076	2.016	1.983
	1.956
800ng/L	1.151	1.157	1.125
	1.163
400ng/L	0.561	0.573	0.54
	0.585
200ng/L	0.271	0.282	0.25
	0.294
100ng/L	0.072	0.121	0.089
	0.17
0ng/L	0.033	0.032	0
	0.032

Precision

We measured random samples of E0037Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	248.1	9.75	3.9
Intra-Assay	2	18	1244.8	39.23	3.2
Intra-Assay	3	18	603	28.98	4.8

Storage	2-8°C

Reference	Growth differentiation factor 15: an emerging diagnostic biomarker of liver fibrosis in chronic hepatitis C patients Journal: Egypt Liver J. 2021;11(1):6.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101773	E0037Hu	96T	$458		Add to Cart
BHE12101773	E0037Hu	48T	$320		Add to Cart

Human Growth Differentiation Factor 15 - GDF15 ELISA Kit

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