Human Granzyme H - GZMH ELISA Kit

Mfr.No.

E5359Hu

Alternative Names

Cathepsin G-like 2;CCP-X;CGL-2;CSP-C;CTLA1;CTSGL2;Cytotoxic serine protease C;Cytotoxic T-lymphocyte proteinase;Granzyme H;GZMH

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27315

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.48ng/L

Background

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.

Gene ID	2999
UniProt ID	P20718
Antigen	GZMH

Research Area

Others

Protocol

These standard curves of E5359Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.653	2.614	2.581
	2.575
600ng/L	1.556	1.539	1.506
	1.522
300ng/L	0.763	0.794	0.761
	0.824
150ng/L	0.454	0.483	0.451
	0.513
75ng/L	0.247	0.243	0.21
	0.238
0ng/L	0.035	0.033	0
	0.03

Precision

We measured random samples of E5359Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	665.86	23.48	3.53
Intra-Assay	2	18	166.51	8.07	4.85
Intra-Assay	3	18	357.74	15.67	4.38

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106722	E5359Hu	48T	$320		Add to Cart
BHE12106722	E5359Hu	96T	$458		Add to Cart

Human Granzyme H - GZMH ELISA Kit

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