Human Gp130/IL6ST ELISA Kit PicoKine®

Mfr.No.

EK0367

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.033	0.099	0.149	0.246	0.431	0.735	1.260	1.910

Description

Human Gp130/IL6ST ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6ST in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;CDw130;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD130;IL6ST;

Molecular Weight

103537 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-E619

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Sgp130

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	186	1115	3384	178	1076	3150
Standard deviation	13.2	73.59	156.6%6	12.63	80.7	198.45
CV (%)	7.1%	6.6%	4.6%	7.1%	7.5%	6.3%

Protein Function

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. .

Background

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein?which is the founding member of the class of all?cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130?is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound?gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	186	203	198	207	198	7.88	3.9%
Sample 2	1115	1150	1073	1056	1098	36.67	3.3%
Sample 3	3384	3507	3537	3330	3439	85.34	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3572
Antigen	Gp130/IL6ST
Gene Name	IL6ST
UniProt ID	P40189
Protein Name	Interleukin-6 receptor subunit beta
Tissue Specificity	Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence	Belongs to the type I cytokine receptor family. Type 2 subfamily.

Applications	ELISA
Research Area	Adapters,Apoptosis,Associated Proteins,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Type Markers,Cytokines,Hypertrophy,Immunology,Intracellular,Signal Transduction,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000380	EK0367	96 wells/kit	$499		Add to Cart

Human Gp130/IL6ST ELISA Kit PicoKine®

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