Human Glypican-5/GPC5 ELISA Kit PicoKine®

Mfr.No.

EK1738

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.073	0.141	0.231	0.332	0.562	0.854	1.451	2.071

Description

Human Glypican-5/GPC5 ELISA Kit PicoKine® (96 Tests). Quantitate Human GPC5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Glypican-5; Secreted glypican-5; GPC5

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-T554

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Glypican-5/GPC5

Subcellular Localization

Cell membrane, GPI-anchor, Extracellular side, Secreted glypican-5, extracellular space

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	226	938	62	243	967
Standard deviation	2.58	11.07	50.7	4.15	16.77	72.5
CV (%)	4.3%	4.9%	5.4%	6.7%	6.9%	7.5%

Protein Function

Cell surface proteoglycan that bears heparan sulfate.

Background

Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	72	63	72	66	5.35	8.1%
Sample 2	226	232	264	250	243	15.00	6.1%
Sample 3	938	925	1033	1044	985	53.83	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2262
Antigen	Glypican-5/GPC5
Gene Name	GPC5
UniProt ID	P78333
Tissue Specificity	In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Applications	ELISA
Research Area	Cytoskeleton/ECM,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001393	EK1738	96 wells/kit	$499		Add to Cart

Human Glypican-5/GPC5 ELISA Kit PicoKine®

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