Human Glypican-5/GPC5 ELISA Kit PicoKine®

Mfr.No.

EK1738

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.073	0.141	0.231	0.332	0.562	0.854	1.451	2.071

Description

Human Glypican-5/GPC5 ELISA Kit PicoKine® (96 Tests). Quantitate Human GPC5 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Glypican-5; Secreted glypican-5; GPC5

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-T554

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Glypican-5/GPC5

Subcellular Localization

Cell membrane, GPI-anchor, Extracellular side, Secreted glypican-5, extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	226	938	62	243	967
Standard deviation	2.58	11.07	50.7	4.15	16.77	72.5
CV (%)	4.3%	4.9%	5.4%	6.7%	6.9%	7.5%

Protein Function

Cell surface proteoglycan that bears heparan sulfate.

Background

Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	72	63	72	66	5.35	8.1%
Sample 2	226	232	264	250	243	15.00	6.1%
Sample 3	938	925	1033	1044	985	53.83	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2262
Gene Name	GPC5
UniProt ID	P78333
Tissue Specificity	In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Applications	ELISA
Research Area	Cytoskeleton/ECM,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001393	EK1738	96 wells/kit	$499		Add to Cart

Human Glypican-5/GPC5 ELISA Kit PicoKine®

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