Human Glycogen Phosphorylase - Liver Form

Mfr.No.

E4620Hu

Sensitivity

0.027ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Glycogen Phosphorylase, Liver Form (also known as PYGL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Glycogen phosphorylase, liver form;PYGL

Molecular Weight

97149

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4620Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.2	0.36	5
Intra-Assay	2	18	11.1	0.28	2.6
Intra-Assay	3	18	2.8	0.12	4.5

Background

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Protocol

These standard curves of E4620Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	1.845	1.875	1.844
	1.904
8ng/ml	1.033	0.982	0.951
	0.932
4ng/ml	0.58	0.538	0.507
	0.496
2ng/ml	0.207	0.253	0.222
	0.298
1ng/ml	0.151	0.124	0.094
	0.097
0ng/ml	0.03	0.031	0
	0.031

Gene ID	5836
UniProt ID	P06737

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105926	E4620Hu	48T	$320		Add to Cart
BHE12105926	E4620Hu	96T	$458		Add to Cart

Human Glycogen Phosphorylase - Liver Form - PYGL ELISA Kit

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