Human Glycine Amidinotransferase - Mitochondrial

Mfr.No.

E3938Hu

Description

This sandwich kit is for the accurate quantitative detection of human Glycine Amidinotransferase Mitochondrial (also known as GATM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GATM;Glycine amidinotransferase, mitochondrial;L-arginine:glycine amidinotransferase;Transamidinase

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

48455

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.26ng/L

Background

Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis.

Gene ID	2628
UniProt ID	P50440
Antigen	GATM

Research Area

Others

Protocol

These standard curves of E3938Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.096	2.185	2.135
	2.274
800ng/L	1.746	1.79	1.74
	1.834
400ng/L	1.311	1.206	1.157
	1.102
200ng/L	0.72	0.723	0.673
	0.725
100ng/L	0.417	0.419	0.37
	0.422
0ng/L	0.048	0.05	0
	0.051

Precision

We measured random samples of E3938Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	295.6	15.78	5.3
Intra-Assay	2	18	872.7	56.92	6.5
Intra-Assay	3	18	180.3	7.79	4.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105372	E3938Hu	48T	$320		Add to Cart
BHE12105372	E3938Hu	96T	$458		Add to Cart

Human Glycine Amidinotransferase - Mitochondrial - GATM ELISA Kit

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