Human Glutaredoxin 1 - GLRX1 ELISA Kit

Mfr.No.

E4698Hu

Description

This sandwich kit is for the accurate quantitative detection of human Glutaredoxin 1 (also known as GLRX1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GLRX1;Glutaredoxin-1;GRX;GRX1;Thioltransferase-1;TTase-1

Reactivity

Human

Detection Range

0.2-70ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11776

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.15ng/ml

Background

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.

Gene ID	2745
UniProt ID	P35754
Antigen	GLRX1

Research Area

Cell Biology

Protocol

These standard curves of E4698Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.332	2.308	2.275
	2.283
20ng/ml	1.729	1.735	1.702
	1.741
10ng/ml	1.265	1.177	1.144
	1.089
5ng/ml	0.74	0.733	0.701
	0.727
2.5ng/ml	0.37	0.404	0.371
	0.439
0ng/ml	0.037	0.033	0
	0.028

Precision

We measured random samples of E4698Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.37	0.66	7
Intra-Assay	2	18	21.56	1.55	7.21
Intra-Assay	3	18	4.29	0.33	7.66

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105990	E4698Hu	48T	$320		Add to Cart
BHE12105990	E4698Hu	96T	$458		Add to Cart

Human Glutaredoxin 1 - GLRX1 ELISA Kit

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