Human Glucose-6-phosphate 1-dehydrogenase

Mfr.No.

E2206Hu

Sensitivity

1.71mU/mL

Detection Range

3-900mU/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

G 6PD;G6PD;Glucose-6-phosphate 1-dehydrogenase

Molecular Weight

59257

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2206Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	385.4	10.16	2.6
Intra-Assay	2	18	165.5	8.15	4.9
Intra-Assay	3	18	275.6	14.27	5.2

Background

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Protocol

These standard curves of E2206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480mU/mL	1.713	1.769	1.733
	1.825
240mU/mL	1.117	1.047	1.01
	0.976
120mU/mL	0.614	0.554	0.517
	0.494
60mU/mL	0.181	0.19	0.153
	0.198
30mU/mL	0.146	0.138	0.102
	0.13
0mU/mL	0.03	0.036	0
	0.043

Gene ID	2539
UniProt ID	P11413

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103746	E2206Hu	48T	$320		Add to Cart
BHE12103746	E2206Hu	96T	$458		Add to Cart

Human Glucose-6-phosphate 1-dehydrogenase - G6PD ELISA Kit

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