Human Glucagon-like Peptide 1 Receptor

Mfr.No.

E3127Hu

Description

This sandwich kit is for the accurate quantitative detection of human Glucagon-like Peptide 1 Receptor (also known as GLP1R) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GLP 1R;GLP-1 receptor;GLP1R;GLP-1R;GLP-1-R;Glucagon-like Peptide 1 receptor

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

53026

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/ml

Background

G-protein coupled receptor for glucagon-like Peptide 1 (GLP-1) (PubMed: 8405712, PubMed: 8216285, PubMed: 7517895, PubMed: 19861722, PubMed: 26308095, PubMed: 27196125, PubMed: 28514449). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed: 8405712, PubMed: 8216285, PubMed: 7517895, PubMed: 19861722, PubMed: 26308095, PubMed: 27196125, PubMed: 28514449). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity).

Gene ID	2740
UniProt ID	P43220
Antigen	GLP1R

Research Area

Others

Protocol

These standard curves of E3127Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.407	2.417	2.387
	2.427
8ng/ml	1.217	1.336	1.306
	1.454
4ng/ml	0.684	0.631	0.601
	0.578
2ng/ml	0.318	0.305	0.275
	0.292
1ng/ml	0.148	0.133	0.103
	0.118
0ng/ml	0.017	0.03	0
	0.044

Precision

We measured random samples of E3127Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.7	0.24	2.4
Intra-Assay	2	18	6.3	0.24	3.7
Intra-Assay	3	18	2.5	0.12	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104659	E3127Hu	48T	$320		Add to Cart
BHE12104659	E3127Hu	96T	$458		Add to Cart

Human Glucagon-like Peptide 1 Receptor - GLP1R ELISA Kit

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