Human GHR/Growth Hormone R ELISA Kit PicoKine®

Mfr.No.

EK1467

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.017	0.036	0.065	0.098	0.174	0.408	1.076	2.127

Description

Human GHR/Growth Hormone R ELISA Kit PicoKine® (96 Tests). Quantitate Human GHR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth hormone receptor;GH receptor;Somatotropin receptor;Growth hormone-binding protein;GH-binding protein;GHBP;Serum-binding protein;GHR;

Molecular Weight

71500 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-Y264

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human GHR

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	353	1151	4591	351	1140	4805
Standard deviation	20.47	66.75	192.82	25.8%7	71.82	201.81
CV (%)	5.8%	5.8%	4.2%	7.2%	6.3%	4.2%

Protein Function

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). .

Background

Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to chromosome 5p13.1-p12. This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization (the receptor may however also exist as a pre-assembled non-functional dimer) and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	353	349	346	304	338	19.78	5.8%
Sample 2	1151	1302	1219	1248	1230	54.47	4.4%
Sample 3	4591	4345	4168	3982	4271	224.72	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2690
Antigen	GHR/Growth
Gene Name	GHR
UniProt ID	P10912
Protein Name	Growth hormone receptor
Tissue Specificity	Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

Applications	ELISA
Research Area	More Gpcr,Neurology Process,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001082	EK1467	96 wells/kit	$499		Add to Cart

Human GHR/Growth Hormone R ELISA Kit PicoKine®

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