Human GHR/Growth Hormone R ELISA Kit PicoKine®

Mfr.No.

EK1467

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.017	0.036	0.065	0.098	0.174	0.408	1.076	2.127

Description

Human GHR/Growth Hormone R ELISA Kit PicoKine® (96 Tests). Quantitate Human GHR in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth hormone receptor;GH receptor;Somatotropin receptor;Growth hormone-binding protein;GH-binding protein;GHBP;Serum-binding protein;GHR;

Molecular Weight

71500 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-Y264

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human GHR

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. On growth hormone binding, GHR is ubiquitinated, intealized, down-regulated and transported into a degradative or non-degradative pathway. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	353	1151	4591	351	1140	4805
Standard deviation	20.47	66.75	192.82	25.8%7	71.82	201.81
CV (%)	5.8%	5.8%	4.2%	7.2%	6.3%	4.2%

Protein Function

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). .

Background

Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to chromosome 5p13.1-p12. This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization (the receptor may however also exist as a pre-assembled non-functional dimer) and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	353	349	346	304	338	19.78	5.8%
Sample 2	1151	1302	1219	1248	1230	54.47	4.4%
Sample 3	4591	4345	4168	3982	4271	224.72	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2690
Gene Name	GHR
UniProt ID	P10912
Protein Name	Growth hormone receptor
Tissue Specificity	Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

Applications	ELISA
Research Area	More Gpcr,Neurology Process,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001082	EK1467	96 wells/kit	$499		Add to Cart

Human GHR/Growth Hormone R ELISA Kit PicoKine®

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