Human Gastrotropin - FABP6 ELISA Kit

Mfr.No.

E5323Hu

Alternative Names

FABP 6;FABP6;Fatty acid-binding protein 6;Gastrotropin;GT;I-15P;I-BABP;ILBP;Ileal lipid-binding protein;Intestinal 15 kDa protein;Intestinal bile acid-binding protein

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14371

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.048ng/ml

Background

Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes (By similarity). In vitro binds to bile acids in the order: deoxycholic acid ; cholic acid ; chenodeoxycholic acid and respective BA conjugation modifies affinities in the order taurine-conjugated ; glycine-conjugated ; unconjugated bile acids. Stimulates gastric acid and pepsinogen secretion (By similarity). (Isoform 2): Essential for the survival of colon cancer cells to bile acid-induced apoptosis.

Gene ID	2172
UniProt ID	P51161
Antigen	FABP6

Research Area

Signal Transduction; Cancer; Metabolism; Cardiovascular

Protocol

These standard curves of E5323Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.987	1.99	1.946
	1.993
12ng/ml	1.369	1.463	1.419
	1.557
6ng/ml	0.651	0.714	0.67
	0.776
3ng/ml	0.418	0.422	0.378
	0.426
1.5ng/ml	0.214	0.205	0.161
	0.196
0ng/ml	0.037	0.044	0
	0.051

Precision

We measured random samples of E5323Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.1	0.57	6.2
Intra-Assay	2	18	10.8	0.42	3.8
Intra-Assay	3	18	4.7	0.23	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106686	E5323Hu	48T	$320		Add to Cart
BHE12106686	E5323Hu	96T	$458		Add to Cart

Human Gastrotropin - FABP6 ELISA Kit

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