Human GAS6 ELISA Kit PicoKine®

Mfr.No.

EK1992

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.034	0.126	0.177	0.292	0.510	0.934	1.536	2.080

Description

Human GAS6 ELISA Kit PicoKine® (96 Tests). Quantitate Human GAS6 in cell culture supeatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth arrest-specific protein 6; GAS-6; AXL receptor tyrosine kinase ligand; GAS6; AXLLG

Immunogen

Expression system for standard: NS0; Immunogen sequence: A49-A678

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human GAS6

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	99	434	1403	119	482	1746
Standard deviation	4.26	28.21	65.94	7.38	33.26	123.97
CV (%)	4.3%	6.5%	4.7%	6.2%	6.9%	7.1%

Protein Function

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. (Microbial infection) Can bridges virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry. Plays a role in Dengue cell entry by apoptotic mimicry. Plays a role in Vaccinia virus cell entry by apoptotic mimicry. Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry.

Background

Growth arrest-specific 6, also known as GAS6, is a human gene coding for the Gas6 protein. It is mapped to 13q34. This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. Gas6 is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	99	92	101	100	98	3.53	3.6%
Sample 2	434	424	411	459	432	17.59	4.0%
Sample 3	1403	1407	1524	1413	1436	50.49	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2621
Gene Name	GAS6
UniProt ID	Q14393
Tissue Specificity	Plasma. Isoform 1 and isoform 2 are widely expressed, isoform 1 being expressed at higher levels than isoform 2 in most tissues. Isoform 2 is the predominant form in spleen.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Hematopoietic Progenitors,Mesenchymal Stem Cells,Myeloid,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Stem Cells,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001498	EK1992	96 wells/kit	$499		Add to Cart

Human GAS6 ELISA Kit PicoKine®

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