Human Gamma Glutamylcyclotransferase

Mfr.No.

E5315Hu

Alternative Names

Cytochrome c-releasing factor 21;Gamma-glutamylcyclotransferase;GGCT

Reactivity

Human

Detection Range

30-9000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21008

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

14.26ng/L

Background

Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis.

Gene ID	79017
UniProt ID	O75223
Antigen	GGCT

Research Area

Cell Biology

Protocol

These standard curves of E5315Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.045	2.068	2.021
	2.091
2400ng/L	1.531	1.545	1.497
	1.559
1200ng/L	0.944	0.861	0.813
	0.777
600ng/L	0.457	0.456	0.408
	0.455
300ng/L	0.24	0.272	0.224
	0.303
0ng/L	0.037	0.048	0
	0.058

Precision

We measured random samples of E5315Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2869.4	148.86	5.2
Intra-Assay	2	18	675.7	33.33	4.9
Intra-Assay	3	18	1483.9	81.38	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106678	E5315Hu	48T	$320		Add to Cart
BHE12106678	E5315Hu	96T	$458		Add to Cart

Human Gamma Glutamylcyclotransferase - GGCT ELISA Kit

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