Human Galectin-9 / Gal 9 ELISA Kit PicoKine®

Mfr.No.

EK1113

Sensitivity

<10 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.075	0.114	0.190	0.263	0.416	0.705	1.022	1.802

Description

Human Galectin-9 / Gal 9 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-9;Gal-9;Ecalectin;Tumor antigen HOM-HD-21;LGALS9;

Molecular Weight

39518 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-T355

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-9

Subcellular Localization

Cytoplasm . Secreted . May also be secreted by a non-classical secretory pathway. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	168	731	3580	177	792	3920
Standard deviation	7.90	51.9	218.38	9.55	66.52	274.4
CV (%)	4.7%	7.1%	6.1%	5.4%	8.4%	7%

Protein Function

Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. .

Background

Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	168	165	163	179	168	6.17	3.6%
Sample 2	731	656	726	619	683	47.37	6.9%
Sample 3	3580	3263	3243	3664	3437	187	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3965
Antigen	Galectin-9
Gene Name	LGALS9
UniProt ID	O00182
Protein Name	Galectin-9
Tissue Specificity	Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
Sequence	Contains 2 galectin domains.

Applications	ELISA
Research Area	Cell Biology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000768	EK1113	96 wells/kit	$499		Add to Cart

Human Galectin-9 / Gal 9 ELISA Kit PicoKine®

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