Human Galectin-8(LGALS8) ELISA kit

Human Galectin-8(LGALS8) ELISA kit

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Mfr.No.

CSB-EL012894HU

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

lectin, galactoside-binding, soluble, 8, Gal-8, PCTA-1, PCTA1, Po66-CBP, Po66 carbohydrate binding protein, galectin 8, galectin-8, galectin-8g, prostate carcinoma tumor antigen 1

Recovery

The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-104
1:400	Average %	95
	Range %	91-99
1:800	Average %	93
	Range %	87-99
1:1600	Average %	86
	Range %	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2400	0.138	0.141	0.140
1200	0.457	0.460	0.459
600	0.903	0.898	0.901
300	1.134	1.121	1.128
150	1.518	1.483	1.501
75	1.689	1.706	1.698
37.5	2.032	2.049	2.041
0	2.187	2.114	2.151

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O00214
Tissue Specificity	Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.

Applications	ELISA
Research Area	LGALS8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502462	CSB-EL012894HU	96T	$695	Add to Cart
BHE10502462	CSB-EL012894HU	5x96T	$3336	Add to Cart
BHE10502462	CSB-EL012894HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.