Human Galectin-7/LGALS7 ELISA Kit PicoKine®

Mfr.No.

EK1286

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.145	0.178	0.211	0.299	0.493	0.819	1.390	2.002

Description

Human Galectin-7/LGALS7 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS7 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-7;Gal-7;HKL-14;PI7;p53-induced gene 1 protein;LGALS7;PIG1;LGALS7B;

Molecular Weight

15075 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-F136

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-7

Subcellular Localization

Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	185	1753	5922	182	1745	5903
Standard deviation	13.13	91.15	236.88	14.37	111.68	277.44
CV (%)	7.1%	5.2%	4%	7.9%	6.4%	4.7%

Protein Function

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. .

Background

Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in coeal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of coeal wounds.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	185	222	208	223	209	15.33	7.3%
Sample 2	1753	1862	1626	1898	1784	106.06	5.9%
Sample 3	5922	5367	5932	6244	5866	315.98	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3963
Gene Name	LGALS7
UniProt ID	P47929
Protein Name	Galectin-7
Tissue Specificity	Mainly expressed in stratified squamous epithelium.
Sequence	Contains 1 galectin domain.

Applications	ELISA
Research Area	Apoptosis,Cancer,Cell Adhesion Proteins,Cell Death,Invasion/Microenvironment,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000875	EK1286	96 wells/kit	$499		Add to Cart

Human Galectin-7/LGALS7 ELISA Kit PicoKine®

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