Human Galectin-4 ELISA Kit PicoKine®

Mfr.No.

EK1275

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.022	0.081	0.169	0.286	0.532	1.151	1.597	1.880

Description

Human Galectin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-4;Gal-4;Antigen NY-CO-27;L-36 lactose-binding protein;L36LBP;Lactose-binding lectin 4;LGALS4;

Molecular Weight

35941 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I323

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-4

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	243	879	64	223	865
Standard deviation	3.24	19.19	45.7	4.28	19.4	47.57
CV (%)	5.4%	7.9%	5.2%	6.7%	8.7%	5.5%

Protein Function

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Background

Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	54	60	64	59	3.57	6%
Sample 2	243	258	258	260	254	6.83	2.6%
Sample 3	879	923	848	978	907	48.89	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3960
Antigen	Galectin-4
Gene Name	LGALS4
UniProt ID	P56470
Protein Name	Galectin-4
Sequence	Contains 2 galectin domains.

Applications	ELISA
Research Area	Cancer,Cell Adhesion,2339,Cytoskeleton/ECM,Energy Metabolism,Energy Transfer Pathways,Epigenetics and Nuclear Signaling,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Tags & Cell Markers,Transcription,Transcription Factors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000865	EK1275	96 wells/kit	$750		Add to Cart

Human Galectin-4 ELISA Kit PicoKine®

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