Human Galectin-3BP ELISA Kit PicoKine®

Mfr.No.

EK1240

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.022	0.094	0.144	0.278	0.520	0.902	1.468	2.070

Description

Human Galectin-3BP ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS3BP in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-3-binding protein;Basement membrane autoantigen p105;Lectin galactoside-binding soluble 3-binding protein;Mac-2-binding protein;MAC2BP;Mac-2 BP;Tumor-associated antigen 90K;LGALS3BP;M2BP;

Molecular Weight

65331 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-D585

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-3BP

Subcellular Localization

Secreted . Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	312	1090	4158	326	1154	3768
Standard deviation	18.72	63.22	282.74	20.86	84.24	271.29
CV (%)	6%	5.8%	6.8%	6.4%	7.3%	7.2%

Protein Function

Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. .

Background

Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cellcell and cellmatrix interactions. Using fluorescence insitu hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	312	367	361	366	351	22.91	6.5%
Sample 2	1090	1067	1143	1041	1085	37.57	3.4%
Sample 3	4158	3836	4350	4159	4125	184.65	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3959
Antigen	Galectin-3BP
Gene Name	LGALS3BP
UniProt ID	Q08380
Protein Name	Galectin-3-binding protein
Tissue Specificity	Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. .

Applications	ELISA
Research Area	Cancer,Cell Cycle,Cell Division,Oncoproteins,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001018	EK1240	96 wells/kit	$399		Add to Cart

Human Galectin-3BP ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Galectin-3BP ELISA Kit PicoKine®

Rate it now..!!