Human Galectin 1 - GAL1 ELISA Kit

Mfr.No.

E2989Hu

Description

This sandwich kit is for the accurate quantitative detection of human Galectin 1 (also known as Gal-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

14 kDa laminin-binding protein;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Gal-1;Galaptin;Galectin-1;HBL;HLBP14;HPL;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;LGALS 1;LGALS1;Putative MAPK-activating protein PM12;S-Lac lectin 1

Reactivity

Human

Detection Range

0.3-90ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14716

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.15ng/ml

Background

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Gene ID	3956
UniProt ID	P09382
Antigen	LGALS1

Research Area

Cell Biology

Protocol

These standard curves of E2989Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.14	2.218	2.165
	2.297
24ng/ml	1.759	1.861	1.808
	1.964
12ng/ml	1.311	1.278	1.225
	1.246
6ng/ml	0.713	0.682	0.628
	0.651
3ng/ml	0.431	0.417	0.363
	0.402
0ng/ml	0.042	0.054	0
	0.066

Precision

We measured random samples of E2989Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6	0.33	5.5
Intra-Assay	2	18	9.3	0.51	5.5
Intra-Assay	3	18	16.2	0.87	5.4

Storage	2-8°C

Reference	Galectin-1 (Gal-1) and Galectin-3 (Gal-3) levels in seminal plasma and serum in azoospermic patients versus fertile men: A cross-sectional study Journal: Arch Ital Urol Androl. 2023 Sep 5;95(3):11462.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104526	E2989Hu	48T	$320		Add to Cart
BHE12104526	E2989Hu	96T	$458		Add to Cart

Human Galectin 1 - GAL1 ELISA Kit

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