Human G Protein-coupled Bile Acid Receptor 1

Mfr.No.

E5310Hu

Sensitivity

0.027ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BG37;GPBAR 1;GPBAR1;G-protein coupled bile acid receptor 1;G-protein coupled receptor GPCR19;hBG37;hGPCR19;M-BAR;Membrane-type receptor for bile acids;

Molecular Weight

35248

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5310Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.7	0.17	3.6
Intra-Assay	2	18	8.5	0.23	2.7
Intra-Assay	3	18	4	0.23	5.8

Background

Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.

Protocol

These standard curves of E5310Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12.8ng/mL	2.05	2.092	2.041
	2.134
6.4ng/mL	1.026	1.027	0.976
	1.028
3.2ng/mL	0.602	0.519	0.468
	0.436
1.6ng/mL	0.198	0.254	0.203
	0.31
0.8ng/mL	0.118	0.112	0.061
	0.106
0ng/mL	0.052	0.051	0
	0.05

Gene ID	151306
UniProt ID	Q8TDU6

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106673	E5310Hu	48T	$320		Add to Cart
BHE12106673	E5310Hu	96T	$458		Add to Cart

Human G Protein-coupled Bile Acid Receptor 1 - GPBAR1 ELISA Kit

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