Human FSTL1/Tsc 36 ELISA Kit PicoKine®

Mfr.No.

EK0965

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.136	0.225	0.325	0.491	0.748	1.117	1.670	2.038

Description

Human FSTL1/Tsc 36 ELISA Kit PicoKine® (96 Tests). Quantitate Human FSTL1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Follistatin-related protein 1;Follistatin-like protein 1;FSTL1;FRP;

Molecular Weight

34986 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-I308

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FSTL1

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	591	1511	9939	616	1368	9532
Standard deviation	26.59	111.81	397.56	38.8	106.7	390.81
CV (%)	4.5%	7.4%	4%	7.8%	7.8%	4.1%

Protein Function

May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity). .

Background

Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	591	609	629	635	616	17.34	2.8%
Sample 2	1511	1639	1624	1771	1636	92.19	5.6%
Sample 3	9939	8661	9621	8867	9272	525.36	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11167
Gene Name	FSTL1
UniProt ID	Q12841
Protein Name	Follistatin-related protein 1
Tissue Specificity	Overexpressed in synovial tissues from rheumatoid arthritis. .
Sequence	Contains 2 EF-hand domains.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cell Biology,Cell Cycle,Cell Differentiation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000731	EK0965	96 wells/kit	$499		Add to Cart

Human FSTL1/Tsc 36 ELISA Kit PicoKine®

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