Human Forkhead Box O4 - FOXO4 ELISA Kit

Mfr.No.

E0629Hu

Alternative Names

AFX;AFX1;Fork head domain transcription factor AFX1;Forkhead box protein O4;FOXO4;MLLT7

Reactivity

Human

Detection Range

20-3500ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

53684

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

8.84ng/L

Background

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Gene ID	4303
UniProt ID	P98177
Antigen	FOXO4

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E0629Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.118	2.102	2.05
	2.086
1000ng/L	1.445	1.402	1.35
	1.359
500ng/L	0.588	0.633	0.58
	0.678
250ng/L	0.364	0.377	0.325
	0.391
125ng/L	0.142	0.138	0.086
	0.134
0ng/L	0.055	0.052	0
	0.05

Precision

We measured random samples of E0629Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	798.38	33.61	4.21
Intra-Assay	2	18	1159.8	30.41	2.62
Intra-Assay	3	18	483.02	14.51	3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102311	E0629Hu	48T	$320		Add to Cart
BHE12102311	E0629Hu	96T	$458		Add to Cart

Human Forkhead Box O4 - FOXO4 ELISA Kit

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