Human FOLR2/Beta Fr ELISA Kit PicoKine®

Mfr.No.

EK1539

Sensitivity

<1 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.028	0.050	0.101	0.207	0.456	0.972	1.941	2.585

Description

Human FOLR2/Beta Fr ELISA Kit PicoKine® (96 Tests). Quantitate Human FOLR2 in cell culture supeatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Folate receptor beta;FR-beta;Folate receptor 2;Folate receptor, fetal/placental;Placental folate-binding protein;FBP;FOLR2;

Molecular Weight

29280 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T17-N230

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FOLR2

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor. Secreted .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	342	1852	4326	361	1834	4010
Standard deviation	23.25	85.49	190.34	29.96	102.7	216.54
CV (%)	6.8%	4.6%	4.4%	8.3%	5.6%	5.4%

Protein Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. .

Background

Folate receptor beta is a protein that in humans is encoded by the FOLR2 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	342	322	342	345	337	9.17	2.7%
Sample 2	1852	1882	1780	2001	1878	79.72	4.2%
Sample 3	4326	4126	3772	4098	4080	198.63	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2350
Gene Name	FOLR2
UniProt ID	P14207
Protein Name	Folate receptor beta
Tissue Specificity	Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues. .

Applications	ELISA
Research Area	Cancer,Cofactors, Vitamins/Minerals,Metabolism,Pathways and Processes,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001262	EK1539	96 wells/kit	$499		Add to Cart

Human FOLR2/Beta Fr ELISA Kit PicoKine®

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