Human Follicular dendritic cell secreted peptide(C4orf7) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL003992HU

Sensitivity

1.95 ng/mL

Detection Range

7.81 ng/mL-500 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chromosome 4 open reading frame 7, FDC-SP, MGC71894, follicular dendritic cell secreted peptide

Recovery

The recovery of rat P?CP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	90	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat P?CP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	88-97
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8NFU4
Tissue Specificity	Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.

Applications	ELISA
Research Area	C4orf7

Storage	Store at 2-8°C. Please refer to protocol.

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