Human FLRT2 ELISA Kit PicoKine®

Mfr.No.

EK1971

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.100	0.191	0.268	0.390	0.867	1.072	1.615	2.137

Description

Human FLRT2 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLRT2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; FLRT2; KIAA0405; UNQ232/PRO265

Immunogen

Expression system for standard: NS0; Immunogen sequence: C36-T660

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FLRT2

Subcellular Localization

Extracellular matrix. Secreted. Endoplasmic reticulum membrane. Microsome membrane. Cell membrane. Single-pass membrane protein. Focal adhesion. Synaptosome.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	45	237	849	54	246	915
Standard deviation	2.16	13.51	56.03	2.75	12.05	58.56
CV (%)	4.8%	5.7%	6.6%	5.1%	4.9%	6.4%

Protein Function

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of c

Background

Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 14q31.3. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	45	54	47	54	50	4.06	8.1%
Sample 2	237	215	225	234	227	8.58	3.7%
Sample 3	849	915	865	819	862	34.77	4.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23768
Gene Name	FLRT2
UniProt ID	O43155
Tissue Specificity	Expressed in pancreas, skeletal muscle, brain, and heart.

Applications	ELISA
Research Area	Growth Factors/Hormones,Mesenchymal Stem Cells,Signal Transduction,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001477	EK1971	96 wells/kit	$499		Add to Cart

Human FLRT2 ELISA Kit PicoKine®

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