Human Filamin A - FLNA ELISA Kit

Mfr.No.

E4396hu

Sensitivity

5.39ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Filamin A (also known as FLNA) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ABP-278;ABP-280 homolog;Actin-binding-like protein;Beta-filamin;Fh1;Filamin homolog 1;Filamin-3;Filamin-B;FLNB;FLN-B;Thyroid autoantigen;Truncated ABP;Truncated actin-binding protein;

Molecular Weight

278164

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4396hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	657.2	25.88	3.9
Intra-Assay	2	18	395.3	23.61	6
Intra-Assay	3	18	162.9	12.73	7.8

Background

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

Protocol

These standard curves of E4396hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.052	2.104	2.065
	2.155
600ng/L	1.221	1.232	1.194
	1.242
300ng/L	0.8	0.739	0.701
	0.678
150ng/L	0.532	0.489	0.451
	0.446
75ng/L	0.191	0.228	0.19
	0.265
0ng/L	0.036	0.038	0
	0.041

Gene ID	2317
UniProt ID	O75369

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105748	E4396hu	48T	$320		Add to Cart
BHE12105748	E4396hu	96T	$458		Add to Cart

Human Filamin A - FLNA ELISA Kit

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