Human Ficolin 2 - FCN2 ELISA Kit

Mfr.No.

E0330Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Ficolin 2 (also known as FCN2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

37 kDa elastin-binding protein;Collagen/fibrinogen domain-containing protein 2;EBP-37;FCN 2;FCN2;Ficolin-2;Ficolin-B;Ficolin-beta;Hucolin;L-ficolin;Serum lectin p35

Molecular Weight

34001

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0330Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.1	0.41	4
Intra-Assay	2	18	2.8	0.15	5.4
Intra-Assay	3	18	4.6	0.16	3.4

Background

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.

Protocol

These standard curves of E0330Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.013	2.102	2.076
	2.191
8ng/ml	1.511	1.443	1.416
	1.375
4ng/ml	0.558	0.606	0.58
	0.654
2ng/ml	0.444	0.414	0.388
	0.385
1ng/ml	0.158	0.152	0.126
	0.146
0ng/ml	0.033	0.026	0
	0.02

Gene ID	2220
UniProt ID	Q15485

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102035	E0330Hu	96T	$458		Add to Cart
BHE12102035	E0330Hu	48T	$320		Add to Cart

Human Ficolin 2 - FCN2 ELISA Kit

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