Human Ficolin-2 ELISA Kit PicoKine®

Mfr.No.

EK1258

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.008	0.101	0.202	0.320	0.768	1.116	1.526	2.050

Description

Human Ficolin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN2 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ficolin-2;37 kDa elastin-binding protein;Collagen/fibrinogen domain-containing protein 2;EBP-37;Ficolin-B;Ficolin-beta;Hucolin;L-ficolin;Serum lectin p35;FCN2;FCNL;

Molecular Weight

34001 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L26-A313

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Ficolin-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	977	6812	22201	979	6528	20934
Standard deviation	44.94	497.27	1354.26	52.86	509.18	1611.91
CV (%)	4.6%	7.3%	6.1%	5.4%	7.8%	7.7%

Protein Function

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .

Background

Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	977	1058	1110	1008	1038	50.5	4.8%
Sample 2	6812	6337	6515	7162	6706	312.97	4.6%
Sample 3	22201	22603	22446	21704	22238	340.22	1.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2220
Gene Name	FCN2
UniProt ID	Q15485
Protein Name	Ficolin-2
Tissue Specificity	Expressed by the liver and secreted in plasma.
Sequence	Belongs to the ficolin lectin family.

Applications	ELISA
Research Area	Cardiovascular,Immunology,Secreted Molecules,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000857	EK1258	96 wells/kit	$450		Add to Cart

Human Ficolin-2 ELISA Kit PicoKine®

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