Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

Mfr.No.

EK1257

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.008	0.110	0.235	0.323	0.620	1.029	1.510	2.030

Description

Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ficolin-1;Collagen/fibrinogen domain-containing protein 1;Ficolin-A;Ficolin-alpha;M-ficolin;FCN1;FCNM;

Molecular Weight

35078 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-A326

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Ficolin-1

Subcellular Localization

Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Found on the monocyte and granulocyte surface.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1355	8333	19299	1365	8931	19839
Standard deviation	88.07	483.31	1042.14	95.55	544.79	1091.14
CV (%)	6.5%	5.8%	5.4%	7%	6.1%	5.5%

Protein Function

Extracellular lectin functioning as a patte- recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular pattes (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed:21037097). Binds preferentially to 9-O-acetylated 2-6- linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. .

Background

Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1355	1460	1601	1587	1500	100.48	6.6%
Sample 2	8333	7592	7727	7941	7898	280.15	3.5%
Sample 3	19299	22441	21939	20805	21121	1207.37	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2219
Gene Name	FCN1
UniProt ID	O00602
Protein Name	Ficolin-1
Tissue Specificity	Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes. .
Sequence	Belongs to the ficolin lectin family.

Applications	ELISA
Research Area	Immunology,Secreted Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000856	EK1257	96 wells/kit	$750		Add to Cart

Human Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®

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